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bodipy493 503  (MedChemExpress)


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    Structured Review

    MedChemExpress bodipy493 503
    Bodipy493 503, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bodipy493+503/BODIPY+493%2F503+methyl+bromide/pmc12969391-192-6-8
    Average 96 stars, based on 31 article reviews
    bodipy493 503 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Knockdown:

    Article Title: SERPINB2 increases endothelial inflammation through augmented fatty acid oxidation to promote choroidal neovascularization.
    Article Snippet: Neovascular age-related macular degeneration (nAMD) is the leading cause of vision loss in the elderly population, with choroidal neovascularization (CNV) being its key pathological feature.. Endothelial cell (EC) inflammation plays a pivotal role in CNV progression.. However, the crucial molecules regulating EC inflammation and the underlying mechanisms remain unclear.

    Incubation:

    Article Title: SERPINB2 increases endothelial inflammation through augmented fatty acid oxidation to promote choroidal neovascularization.
    Article Snippet: Neovascular age-related macular degeneration (nAMD) is the leading cause of vision loss in the elderly population, with choroidal neovascularization (CNV) being its key pathological feature.. Endothelial cell (EC) inflammation plays a pivotal role in CNV progression.. However, the crucial molecules regulating EC inflammation and the underlying mechanisms remain unclear.

    Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques
    Article Snippet: Sections were sealed and mounted using an anti-fluorescence quencher (S2100, Solarbio). .. Cells or sections were incubated with BODIPY493/503 (HY-D1614, MedChemExpress) in the dark for 30 min, then processed for immunofluorescence as previously described. ..

    Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques.
    Article Snippet: Sections were sealed and mounted using an antifluorescence quencher (S2100, Solarbio). .. Cells or sections were incubated with BODIPY493/503 (HY-D1614, MedChemExpress) in the dark for 30 min, then processed for immunofluorescence as previously described. ..

    Staining:

    Article Title: SERPINB2 increases endothelial inflammation through augmented fatty acid oxidation to promote choroidal neovascularization.
    Article Snippet: Neovascular age-related macular degeneration (nAMD) is the leading cause of vision loss in the elderly population, with choroidal neovascularization (CNV) being its key pathological feature.. Endothelial cell (EC) inflammation plays a pivotal role in CNV progression.. However, the crucial molecules regulating EC inflammation and the underlying mechanisms remain unclear.

    Article Title: Isorhamnetin Ameliorates Non-Esterified Fatty Acid-Induced Apoptosis, Lipid Accumulation, and Oxidative Stress in Bovine Endometrial Epithelial Cells via Inhibiting the MAPK Signaling Pathway.
    Article Snippet: .. Lipid droplet formation was assessed using Oil Red O (Solarbio, Beijing, China) and BODIPY493/503 (MedChemExpress, Shanghai, China) staining techniques. ..

    Article Title: Lysophosphatidylethanolamine 18:1 drives clear cell renal cell carcinoma by stabilizing SIRT6 to reprogram lipid metabolism
    Article Snippet: Lipid droplets were visualized under a light microscope (Leica DM6000B), and the optical density was quantified at 510 nm. .. The cells were fixed and stained with BODIPY493/503 (5 μM, MCE) or Nile Red (1:200, Solarbio) for 30 min. , Nuclei were counterstained with DAPI. .. The fluorescence intensity was analyzed via ImageJ, and images were acquired with a Leica TCS SP8 confocal microscope.

    Article Title: 18β-Glycyrrhetinic acid mitigates adverse effects of high-fat diets on liver fibrosis in largemouth bass ( Micropterus salmoides ) by reducing mitochondrial Ca 2+ level
    Article Snippet: .. Additionally, primary hepatocytes were stained using BODIPY493/503 (HY-W090090, MedChemExpress, Monmouth Junction, NJ, USA) for 20 min and 4′,6-diamidino-2-phenylindole (DAPI) (C1006, Beyotime Biotechnology, Shanghai, China) for 5 min. Fluorescent images were obtained using a fluorescence microscope (BX53, Olympus Corporation, Tokyo, Japan). ..

    Article Title: Lysophosphatidylethanolamine 18:1 drives clear cell renal cell carcinoma by stabilizing SIRT6 to reprogram lipid metabolism.
    Article Snippet: Images were captured via a Leica confocal microscope.57 Oil red O staining The cells or frozen tissue sections were fixed with 4% paraformaldehyde, incubated with Oil Red O working solution (Solarbio, G1263) for 15min, and counterstained with hematoxylin. .. Lipid droplets were visualized under a light microscope (Leica DM6000B), and the optical density was quantified at 510 nm.56 BODIPY493/503 staining and Nile red staining The cells were fixed and stained with BODIPY493/503 (5 μM, MCE) or Nile Red (1:200, Solarbio) for 30 min.63,64 Nuclei were counterstained with DAPI. .. The fluorescence intensity was analyzed via ImageJ, and images were acquired with a Leica TCS SP8 confocal microscope.

    Article Title: Isorhamnetin Ameliorates Non-Esterified Fatty Acid-Induced Apoptosis, Lipid Accumulation, and Oxidative Stress in Bovine Endometrial Epithelial Cells via Inhibiting the MAPK Signaling Pathway
    Article Snippet: .. Lipid droplet formation was assessed using Oil Red O (Solarbio, Beijing, China) and BODIPY493/503 (MedChemExpress, Shanghai, China) staining techniques. ..

    Immunofluorescence:

    Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques
    Article Snippet: Sections were sealed and mounted using an anti-fluorescence quencher (S2100, Solarbio). .. Cells or sections were incubated with BODIPY493/503 (HY-D1614, MedChemExpress) in the dark for 30 min, then processed for immunofluorescence as previously described. ..

    Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques.
    Article Snippet: Sections were sealed and mounted using an antifluorescence quencher (S2100, Solarbio). .. Cells or sections were incubated with BODIPY493/503 (HY-D1614, MedChemExpress) in the dark for 30 min, then processed for immunofluorescence as previously described. ..

    Fluorescence:

    Article Title: 18β-Glycyrrhetinic acid mitigates adverse effects of high-fat diets on liver fibrosis in largemouth bass ( Micropterus salmoides ) by reducing mitochondrial Ca 2+ level
    Article Snippet: .. Additionally, primary hepatocytes were stained using BODIPY493/503 (HY-W090090, MedChemExpress, Monmouth Junction, NJ, USA) for 20 min and 4′,6-diamidino-2-phenylindole (DAPI) (C1006, Beyotime Biotechnology, Shanghai, China) for 5 min. Fluorescent images were obtained using a fluorescence microscope (BX53, Olympus Corporation, Tokyo, Japan). ..

    Microscopy:

    Article Title: 18β-Glycyrrhetinic acid mitigates adverse effects of high-fat diets on liver fibrosis in largemouth bass ( Micropterus salmoides ) by reducing mitochondrial Ca 2+ level
    Article Snippet: .. Additionally, primary hepatocytes were stained using BODIPY493/503 (HY-W090090, MedChemExpress, Monmouth Junction, NJ, USA) for 20 min and 4′,6-diamidino-2-phenylindole (DAPI) (C1006, Beyotime Biotechnology, Shanghai, China) for 5 min. Fluorescent images were obtained using a fluorescence microscope (BX53, Olympus Corporation, Tokyo, Japan). ..

    Light Microscopy:

    Article Title: Lysophosphatidylethanolamine 18:1 drives clear cell renal cell carcinoma by stabilizing SIRT6 to reprogram lipid metabolism.
    Article Snippet: Images were captured via a Leica confocal microscope.57 Oil red O staining The cells or frozen tissue sections were fixed with 4% paraformaldehyde, incubated with Oil Red O working solution (Solarbio, G1263) for 15min, and counterstained with hematoxylin. .. Lipid droplets were visualized under a light microscope (Leica DM6000B), and the optical density was quantified at 510 nm.56 BODIPY493/503 staining and Nile red staining The cells were fixed and stained with BODIPY493/503 (5 μM, MCE) or Nile Red (1:200, Solarbio) for 30 min.63,64 Nuclei were counterstained with DAPI. .. The fluorescence intensity was analyzed via ImageJ, and images were acquired with a Leica TCS SP8 confocal microscope.



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